Mass Spectrometry Facility

Examples of approach / data generated for various proteomic projects

MassSpec Example

Post-Translational Modification

Identification of ubiquitination sites: Iyengar P, Jaynes P, Rodon L, Lama D, Law KP, Lim YP, Verma C, Seoane J, and Eichhorn P. USP15 regulates SMURF2 kinetics through C-lobe mediated deubiquitination. 2015 Sci. Rep. 5:14733

Identification of phosphorylation sites: Assidiq BF, Tan KY, Toy W, Chong PK and Lim YP. EGFR S1166 phosphorylation induced by a combination of EGF and gefitinib has a potentially negative impact on lung cancer cell growth. 2012 J. Proteome Res. 11(8):4110-9

Targeted Proteomics

Phosphoproteomics: Chen Y, Choong LY, Lin QS, Philps, R, Wong CH, Ang BK, Loh MCS, Hew CL, Shah, N, Druker BJ, Chong PK and Lim YP. Differential expression of novel tyrosine kinase substrates during breast cancer development. 2007 Mol. Cell. Proteomics 6(12):2072-87

Phosphoproteomics: Chen YH*, Low TY*, Choong LY, Rajarshi Sankar Ray, Tan YL, Toy Weiyi, Lin QS, Ang BK, Wong CH, Lim Simin, Li B, Hew CL, Sze NSK, Druker BJ and Lim YP. Phosphoproteomics identified Endofin, DCBLD2 and KIAA0582 as novel targets of EGF signaling and Gefitinib in human cancer cells. 2007 Proteomics 7, 2384–2397

Membrane Proteomics: Goh WQJ, Ow GS, Kuznetsov V, Chong PK, Lim YP. DLAT Subunit of the Pyruvate Dehydrogenase Complex is Upregulated in Gastric Cancer – implications in cancer therapy. 2015 Am J Transl. Res. 7(6):1140-1151

Membrane Proteomics: Yang YX, Toy WY, Choong LY, Hou PL, Ashktorab H, Smoot DT, Yeoh KG and Lim YP. Discovery of SLC3A2 cell membrane protein as a potential gastric cancer biomarker – implications in molecular imaging. 2012 J. Proteome Res. Dec 7;11(12):5736-47

Secretomics: See LP, Lu S, Chong PK and Lim YP. Increased secretion of CXCL3 as breast cancer cells acquire metastasis potential. 2014 Current Cancer Drug Target Mar;14(3):294-309

Plasma Proteomics

Using human plasma: Chong PK, Lee HY, Loh MCS, Lin Q, So J, Lim KH, Soo RA, Yong WP, Chan SP, Smoot DT, Hassan A, Yeoh KG and Lim YP. Up-regulation of plasma C9 protein in gastric cancer patients. 2010 Proteomics 10(18):3210-21.

Using animal model: Chong, PK, Lee, HY, Zhou J, Liu SC, Loh, MCS, Wang TT, Chan SP, Smoot DT, Ashktorab H, So, JBY, Lim KH, Yeoh KG and Lim YP. ITIH3 is a potential biomarker for early detection of gastric cancer. 2010 J. Proteome Res. 9(7)-3671–3679

Using animal model: Chong PK, Lee HY, Zhou J, Liu SC, Loh MCS, So JBY, Lim KH, Yeoh KG and Lim YP. Reduced plasma APOA1 level is associated with Gastric Tumor Growth in MKN45 mouse xenograft model. 2010 J. Proteomics 73(8):1632-1640

Shot-Gun Proteomics

Ophthalmology: Hou A, Lan W, Law KP, Khoo SC, Tin MC, Lim YP, Tong L. Evaluation of global differential gene and protein expression in primary pterygium: S100A8 and S100A9 as possible drivers of a signaling network. 2014 PLoS One 13;9(5):e97402

Bio-Processing: Lim UM, Yap MGS , Lim YP, Goh LT, Ng SK. Identification of Autocrine Growth Factors Secreted by CHO cells for Applications in Single Cell Cloning Media. 2013 J Proteome. Res. Jul 2013; 12(7): 3496-510

Cancer: Choong LY, Lim S, Chong PK, Wong CY, Shah N and Lim YP. Proteome-wide profiling of the MCF10AT breast cancer progression model. 2010 PLoS One Jun 9;5(6):e11030

Cancer: Lim S, Choong LY, Chong PK, Chen Y and Lim YP. Regulation of Macrophage Inhibitory Factor (MIF) by Epidermal Growth Factor Receptor (EGFR) in the MCF10AT model of breast cancer progression. 2009 J. Proteome Res. 8(8): 4062-4076

Cancer: Ho J, Kong WFJ, Choong LY*, Loh MCS, Toy W, Chong PK, Wong CH, Wong CY, Shah N and Lim YP. Novel Breast Cancer Metastasis-associated proteins. 2009 J. Proteome Res. 8(2):583-594