Marie-Veronique CLEMENT

Associate Professor

+65 6516 7985
bchmvc@nus.edu.sg

Affiliations

Associate Professor, Department of Biochemistry, Yong Loo Lin School of Medicine, NUS.
Assistant Dean (Student Affairs), Yong Loo Lin School of Medicine, NUS.
Supervisor, NUS Graduate School for Integrative Sciences and Engineering (NGS), NUS.

Biodata

Dr. Marie-Veronique Clement obtained her basic degree in Cellular Biology (B.Sc) with major in Immunology from Paris-6 University, Paris, France in 1986. She pursued her interest in Immunology during her M.Sc (1987) and PhD (1991) at the Pasteur Institute and Paris-6 University, Paris, France. Following her doctoral studies, she proceeded to the United States (1992-1996) as a post-doctoral fellow in the Department of Pathology at Harvard Medical School/Massachusetts General Hospital, Boston, MA. It was during her post-doctoral training that she made the significant observation that, contrary to the common belief; an increase in intracellular level of superoxide could inhibit death receptor-induced apoptotic signaling (Clement and Stamenkovic, 1996). She joined the National University Medical Institute, National University of Singapore in 1997 as a Research Associate, was appointed as an Assistant Professor in the Department of Biochemistry, NUS in 2001 and is currently a tenured Associate Professor in the Dept. of Biochemistry as well as the Deputy Head of the Department of Biochemistry and an Assistant Dean Education (Student affairs) at the Yong Loo Lin School of Medicine. As an independent PI, Dr Clement’s group dispelled the dogmatic view of reactive oxygen species as only toxic molecules by providing evidence for their involvement in cell survival signaling pertinent to carcinogenesis. For this work she was the Recipient of the NUS Outstanding Research Award (team award) 2005. More recently, Dr Clement’s group has made significant progress in the understanding of the pathway involved in he induction of cell survival by superoxide. In particular her group is the first involving the regulation of the Na+/H+ exchanger 1 expression in the redox control of cell survival and cell death. Finally, her most recent contribution is the demonstration that an increase in intracellular level of superoxide contributes to the regulation of the oncogenic kinase Akt through an S-nitrosylation mediated inhibition of the tumor suppressor PTEN and inhibition of the dephosphorylation of cytosolic Akt by PP2A.

Research Interest

* Reactive oxygen species and regulation of cell survival and cell death.
The first interest of our research group is to understand the role of intracellular reactive oxygen species such as superoxide (O2.-) and hydrogen peroxide (H2O2) in the regulation of apoptosis and the regulation of cell response to death triggers.

* Superoxide dependent survival pathways in tumor cells.
Our second interest is to assess if intracellular production of O2.- could be a common denominator to oncogene associated survival pathways in tumor cell. A better understanding of these O2.-.-producing pathway(s) may be of critical importance to improve our understanding of cancer development, and most importantly may help to design new approaches to cancer therapy.

Selected Publications

  1. Clément MV Stamenkovic, I. Superoxide anion is a natural inhibitor of FAS-mediated cell death. Embo J. 15:216-225; 1996

  2. Akram, S.; Teong, H. F.; Fliegel, L.; Pervaiz, S.; Clément MV. Reactive oxygen species-mediated regulation of the Na+-H+ exchanger 1 gene expression connects intracellular redox status with cells' sensitivity to death triggers. Cell Death Differ 13:628-641; 2006.

  3. Pervaiz, S.; Clément MV. Superoxide anion: oncogenic reactive oxygen species? Int J Biochem Cell Biol 39:1297-1304; 2007.

  4. Kumar, A. P.; Quake, A. L.; Chang, M. K.; Zhou, T.; Lim, K. S.; Singh, R.; Hewitt, R. E.; Salto-Tellez, M.; Pervaiz, S.; Clément MV. Repression of NHE1 expression by PPARgamma activation is a potential new approach for specific inhibition of the growth of tumor cells in vitro and in vivo. Cancer Res 69:8636-8644; 2009.

  5. Velaithan, R.; Kang, J.; Hirpara, J. L.; Loh, T.; Goh, B. C.; Le Bras, M.; Brenner, C.; Clément MV.; Pervaiz, S. The small GTPase Rac1 is a novel binding partner of Bcl-2 and stabilizes its antiapoptotic activity. Blood 117:6214-6226; 2011.

  6. Loo, S. Y.; Chang, M. K.; Chua, C. S.; Kumar, A. P.; Pervaiz, S.; Clément MV. NHE-1: a promising target for novel anti-cancer therapeutics. Curr Pharm Des 18:1372-1382; 2012.

  7. Luo, L.; Kaur Kumar, J.; Clément MV. Redox control of cytosolic Akt phosphorylation in PTEN null cells. Free Radic Biol Med 53:1697-1707; 2012.

  8. Koh, L. W.; Koh, G. R.; Ng, F. S.; Toh, T. B.; Sandanaraj, E.; Chong, Y. K.; Phong, M.; Tucker-Kellogg, G.; Kon, O. L.; Ng, W. H.; Ng, I. H.; Clément MV.; Pervaiz, S.; Ang, B. T.; Tang, C. S. A Distinct Reactive Oxygen Species Profile Confers Chemoresistance in Glioma-Propagating Cells and Associates with Patient Survival Outcome. Antioxid Redox Signal; 2013 Dec 20;19(18):2261-79.

  9. Kumar AP, Loo SY, Shin SW, Tuan ZT, Chon BE, Singh R, Putti TC, Ong CW, Salto-Tellez M, Goh BC, Park JI, Thiery JP, Pervaiz S, Clément MV. Targeting MnSOD in Basal Breast Carcinoma using Agonists of PPARγ: a new strategy for enhancing chemosensitivity. Antioxid Redox Signal 2014 May 20;20(15):2326-46.

  10. Benjamin M. Gyori, Gireedhar Venkatachalam, P.S. Thiagarajah, David Hsu, Marie-Veronique CLEMENT. OpenComet: An automated tool for comet assay image analysis. Redox Biology 2014 Jan 9;2:457-65.2014