Ee Sin CHEN

Assistant Professor

+65 6516 5616
bchces@nus.edu.sg

Affiliations

Assistant Professor, Department of Biochemistry, Yong Loo Lin School of Medicine, NUS.
Supervisor, NUS Graduate School for Integrative Sciences and Engineering (NGS), NUS.
Editorial Board, Scientific Reports.
Associate Editor, Gene.

Education

Degree and Institution Year(s)
BSc (Hons): National University of Singapore (NUS), Faculty of Science 1992
M.Sc.: Kyoto University, Faculty of Science, Japan 1999
Ph.D.: Kyoto University, Faculty of Science, Japan 2003

Professional Experience

Position and Institute Year(s)
Assistant Professor, Department of Biochemistry, Yong Loo Lin School of Medicine, NUS 2009–present
Post-doctoral Research Fellow, National Cancer Institute, National Institutes of Health, USA 2004–2009
Japan Society for the Promotion of Science Post-doctoral Fellow, Kyoto University, Japan 2003–2004
Assistant Research Officer, Institute of Molecular and Cell Biology, Singapore 1992–1996

Research Interest

Maintaining the integrity of the genome is essential for the survival of a cell and genomic instability is associated with many human diseases including cancers. A cell ensures the integrity of its genome with an intricate interplay of epigenetic mechanisms to safeguard nuclear processes, including chromosome segregation, repair of DNA damage lesions and gene expression. Our lab aims to investigate the epigenetic mechanisms at the molecular level by:

* Identifying and characterizing factors that epigenetically control essential chromatin functions that have direct implication to genomic stability.
* Elucidating how different epigenetic mechanisms interplay to control faithful inheritance of chromatin information through the cell cycle.
* Investigating the genetic and epigenetic regulation of functional sub-nuclear compartments.

Our lab employs the powerful genetics, cytology and biochemistry of the model organism fission yeast to address these important issues. Results obtained will be extended to elucidate epigenetic mechanisms operating in the mammalian cells, to facilitate the development of therapy for human diseases and cancers.

Selected Publications

  1. Jackson R.A., Wu J.S., and Chen E.S. (2016) C1D family proteins in coordinating RNA processing, chromosome condensation and DNA damage response. Cell Division [Accepted].

  2. Nguyen T.T., Lim Y.J., Fan M.H.M., Jackson R.A., Lim K.K., Ang W.H., Ban K.H., and Chen E.S. (2016) Calcium modulation of doxorubicin cytotoxicity in yeast and human cells. Genes to Cells 21, 226-240.

  3. Jackson R.A., and Chen E.S. (2016) Synthetic lethal approaches for assessing combinatorial efficacy of chemotherapeutic drugs. Pharmacology & Therapeutics doi: 10.1016/j.pharmthera.2016.01.014 [Epub ahead of print].

  4. Nguyen T.T., Chua J.K.K., Seah K.S., Koo S.H., Yee J.Y., Yang E.G., Lim K.K., Pang S.Y.W., Yuen A., Zhang L., Ang W.H., Dymock B., and Chen E.S. (2016) Predicting chemotherapeutic drug combinations through gene network profiling. Scientific Reports 5, 18658.

  5. Li C., Zhang Y., Yang Q., Ye F., Sun S.Y., Chen E.S., and Liou Y.C. (2016) NuSAP modulates the dynamics of kinetochore microtubules by attenuating MCAK depolymerisation activity. Scientific Reports 6, 18773

  6. Lim K.K., Ong T.Y., Tan Y.R., Yang E.G., Ren B.B., Seah K.S., Yang Z., Tan T.S., Dymock B.W., and Chen E.S. (2015) Mutation of histone H3 serine 86 disrupts GATA factor Ams2 expression and precise chromosome segregation in fission yeast. Scientific Reports 5, 15064.

  7. Tang M.Y., Guo H., Nguyen T.T., Low L.S., Jackson R.A., Yamada T. and Chen E.S. (2015) Two fission yeast high mobility group box proteins in the maintenance of genomic integrity following doxorubicin insult. Gene 562, 70-75.

  8. Nguyen T.T., Lim J.S., Tang R.M., Zhang L. and Chen E.S. (2015) Fitness profiling links topoisomerase II regulation of centromeric integrity to doxorubicin resistance in fission yeast. Scientific Reports 5, 8400.

  9. Tay Z., Koo S.H., Nguyen T.T., Tan T.S., Chen M.L., Chin C.F., Lim K.K., Ang W.H., Bay B.H., Lee E.J., and Chen E.S. (2014) P-glycoprotein and vacuolar ATPase synergistically confer anthracycline resistance to fission yeast and human cells. Current Medicinal Chemistry 21, 251-260.

  10. Tay Z., Eng R.J., Sajiki K., Lim K.K., Tang M.Y., Yanagida M., and Chen E.S. (2013) Cellular robustness conferred by genetic crosstalk underlies resistance against chemotherapeutic drug doxorubicin in fission yeast. PLoS ONE vol 8, e55041.

  11. Reyes-Turcu F.E., Zhang K., Zofall M., Chen E.., and Grewal S.I.S. (2011) Defects in RNA quality control factors reveal RNAi-independent nucleation of heterochromatin. Nature Structural & Molecular Biology 18, 1132-1138.

  12. Cam H.P., Chen E.S., and Grewal S.I.S. (2009) Transcriptional scaffolds for heterochromatin assembly. Cell 136, 610-614

  13. Chen E.S., K. Zhang, Nicholas E., Cam H.P., Zofall M., and Grewal S.I.S. (2008) Cell cycle control of centromeric repeat transcription and heterochromatin assembly. Nature 451, 734-737.

  14. Murakami H., Goto D.B., Toda T., Chen E.S., Grewal S.I., Martienssen R.A. and Yanagida M. (2007) Ribonuclease activity of Dis3 is required for mitotic progression and provides a possible link between heterochromatin and kinetochore function. PLoS ONE 2, e317.

  15. Cam H.P., Sugiyama T., Chen E.S., Chen X., FitzGerald P.C., and Grewal S.I.S. (2005) Comparative analysis of heterochromatin- and RNAi-mediated epigenetic control of the fission yeast genome. Nature Genetics 37, 809-819.

  16. Chen E.S., Sutani T., and Yanagida M. (2004) Cti1/C1D interacts with condensin SMC hinge and supports the DNA repair function of condensin. Proceedings of the National Academy of Sciences USA 101, 8078-8083.

  17. Chen E.S., Yanagida M., and Takahashi K. (2003) Does a GATA factor makes the bed for centromeric nucleosomes? Cell Cycle 2, 277-278.

  18. Chen E.S., Saitoh S., Yanagida M., and Takahashi K. (2003) A cell cycle-regulated GATA factor promotes centromeric localization of CENP-A in fission yeast. Molecular Cell 11, 175-187.

  19. Takahashi K., Chen E.S., and Yanagida M. (2000) Requirement of Mis6 centromere connector for localizing a CENP-A-like protein in fission yeast. Science 288, 2215-2219.